Phosphoribosyl Pyrophosphate Amidotransferase

Properties of 5-phosphoribosyl-1-pyrophosphate amidotransferase from human lymphoblasts.

The Inhibition of Phosphoribosyl-pyrophosphate Amidotransferase Activity by Cordycepin Monophosphate.

I. Cordycepin monophosphate has been shown to inhibit the activity of phosphoribosyl-pyrophosphate amidotransferase (ribosylamine-5-phosphate:pyrophosphate phosphoribosyltransferase (ghitamate-amidating), EC 2.4.2.14) obtained from both pigeon liver and Bacillus subtilis. 2. Evidence is presented for the existence of a phosphorylated derivative of cordycepin in Bacillus subtilis cells which wer...

Hepatic phosphoribosyl pyrophosphate concentration

Measurements have been made of the tissue content of phosphoribosyl pyrophosphate (PPRibP) and of a range of metabolic intermediates involved in the energy charge of the cell, the glycolytic and pentose phosphate pathways, and of the activity of the enzymes of the pentose phosphate pathway and of PPRibP synthetase (EC 2.7.6.1) in the livers of normal, diabetic, insulin-treated diabetic and star...

Concentration of phosphoribosyl pyrophosphate in renal hypertrophy

Studies were made of the renal phosphoribosyl pyrophosphate (PPRibP) content and PPRibP synthetase (EC 2.7.6.1) activity in rats diabetic for 5, 14 or 20 days, or unilaterally nephrectomized (UN) for 5 days, and in doubly lesioned animals. Approximately equal degrees of renal enlargement were found after 5 days diabetes or 5 days UN. In the doubly lesioned animals the increment of growth was ad...

Altered 5-phosphoribosyl 1-pyrophosphate amidotransferase activity in 6-thioguanine-resistant HL-60 human acute promyelocytic leukemia cells.

A novel mechanism of resistance to the antileukemic agent 6-thioguanine (TGua) was demonstrated in a clone (TGuo-30-2) derived from HL-60 human acute promyelocytic leukemia cells. The clone was isolated by prescreening mutagenized HL-60 cells in hypoxanthine-amethopterin-thymidine medium, followed by selection with 6-thioguanosine. TGuo-30-2 cells were cross-resistant to TGua and beta-2'-deoxyt...